Functional Genomics, Proteomics & Metabolomics Facility Core

Terrance Kavanagh, Director
Yvonne Lin, Co-Director, Metabolomics

Contact PersonTheo Bammlertbammler@uw.edu,  206-616-7378

Services:

  • Global transcriptome and miRNA profiling using microarrays
  • Global transcriptome profiling using massively parallel sequencing (RNA-seq)
  • Targeted mRNA, microRNA, and lincRNA gene expression profiling (low and medium throughput)
  • Single nucleotide polymorphism (SNP) analysis using microarrays
  • Targeted genotyping of single nucleotide polymorphisms (SNPs)
  • Targeted genotyping of SNPs in Drug Metabolism Enzymes and Transporter genes (DMET)
  • Targeted genotyping of SNPs for ancestry characterization
  • Methylation profiling using targeted enrichment, bisulfite conversion, and massively parallel sequencing
  • Targeted DNA methylation profiling
  • Genome-wide copy number variation survey using microarrays
  • Targeted copy number variation analysis
  • In situ hybridization using QuantiGene ViewRNA Assays for mRNA and microRNA
  • Support preparation and review of manuscripts and grant proposals
  • Basic proteomic services include:
  • Consultation on experimental design, including sample preparation
  • Assistance with finding collaborators to perform discovery proteomics
  • Assistance with getting experiments addressed at UW Proteomics Centers
  • Typical mass spec runs
  • Investigations on post-translational modifications
  • Mass spec based peptide quantifications (e.g. SIM and MRM methods)
  • Consultation on data analysis
  • Consultation on protein validation by ELISA, Luminex (fluorescent bead- or magnetic bead-based), or TaqMan assays
  • Perform protein validation by ELISA, Luminex (fluorescent beador magnetic bead-based), or TaqMan assays
  • Facilitating metabolomics analysis