David L. Eaton, PhD

Dean, Graduate School (Primary department)
Professor, Env. and Occ. Health Sciences (Primary department)
Dr. Eaton received his Ph.D. in pharmacology from the University of Kansas Medical Center (KUMC) in 1978. Following a post-doctoral fellowship in Toxicology at KUMC, he joined the faculty of the University of Washington in 1979. He served as Toxicology Program Director in the Department of Environmental Health from 1983-90, then as Associate Chairman of the Department from 1990-92, as Associate Dean for Research in the School of Public Health from 1999-2005, and Associate Vice Provost for Research from 2005-2013. He is currently Dean and Vice Provost of the Graduate School at the University of Washington. He continues has academic appointment as Professor Environmental and Occupational Health Sciences, and Public Health Genetics. He served as the founding director of the Center for Ecogenetics & Environmental Health, an NIEHS Core Center of Excellence, from 1995 – 2013. After assuming the role of Dean and Vice Provost of the Graduate School, Dr. Eaton stepped down as Director of the CEEH, although he remains active as the Associate Director.. Nationally, he has served on the Board of Directors and as Treasurer of the American Board of Toxicology (1990-94), and as President of the Society of Toxicology (2001-02). He has also served on the Board of Environmental Studies and Toxicology, National Academy of Sciences /National Resource Council (1996-99), as a member of the Board of Directors and Vice-President of the Toxicology Education Foundation, and on the Board of Trustees of the Academy of Toxicological Sciences. He is an Elected Fellow of the American Association for the Advancement of Science and the Academy of Toxicological Sciences. In 2011 he was elected to the Institute of Medicine of the National Academy of Sciences, and to the Washington State Academy of Sciences. He has published over 150 scientific articles and book chapters in the field of toxicology and risk assessment.

Contact Information

University of Washington
Office: G-1 Communications Building
Box: 353770
Department of Environmental and Occupational Health Sciences
Seattle, WA 98105-6099
Tel: 206-685-3785
Tel: 206-543-5900
Fax: 206-685-3234

Research Interests

  • Biochemical toxicology, aflatoxin carcinogenesis, glutathione-mediated biotransformation of toxic chemicals, genetic susceptibility to environmental carcinogens, 'gene-environment' interactions
  • KEYWORDS: Bioactivation/Carcinogenesis, bioinformatics, Drug metabolism, Ethics, Legal, and Social Issues (ELSI), Food additives & contaminants; Genetic susceptibility to toxins; In vitro toxicologyToxicogenomics

Teaching interests

Toxicogenomics, pharmacogenomics, basic principles of toxicology

Education

PhD, Pharmacology and Toxicology, University of Kansas Medical Center, 1978

Projects

Program/Grant Name: Center for Ecogenetics and Environmental Health
Funding Agency: NIH/NIEHS (P30 ES07033)
Many diseases of public health importance, such as most cancers, Alzheimer's Disease, Parkinson's disease, asthma, and even birth defects, arise through a complex interaction of genetics and environment. Investigators in the Center for Ecogenetics and Environmental Health are conducting research to understand how specific genetic traits increase or decrease an individual's likelihood of contracting a chronic disease or illness. For example, researchers are studying how genetic differences in the way people break down (metabolize) drugs and other chemicals might increase their chances of getting cancer when exposed to potentially cancer-causing chemicals in their workplace, diet or lifestyle activities (e.g., smoking). Other researchers in the Center are studying genetic traits that, when combined with exposure to substances in the diet or general environment, make individuals more likely to develop chronic neurological diseases such as Parkinson's disease. Other researchers are investigating the link between air pollution and cardiovascular disease in genetically susceptible populations, and environmental factors that might cause birth defects in genetically susceptible individuals. In addition to basic research, the Center includes community engagement activities that include consideration of the ethical, legal and social issues related to the use, and potential misuse, of genetic susceptibility information. The Community Outreach and Ethics Core within the CEEH develops workshops and educational materials, and promotes community education, in the environmental health sciences. Although based in the School of Public Health and Community Medicine, CEEH Investigators come from a variety of schools and departments across the University. Research findings from CEEH investigators will help unravel how genetics and environment interact to produce disease, and are an important extension of the exciting new information coming from the Human Genome Project.

Program/Grant Name: In vitro toxicology on engineered nanomaterials
Funding Agency: NIH/NIEHS (U19)
Rapid advances in nanotechnology have resulted in the generation of a wide variety of engineered nanomaterials (ENMs). There is an increasing and urgent need to evaluate the possible adverse effects of ENMs on human health and the environment, and this in turn requires the development and validation of in vitro test systems suitable for nanomaterials testing that are predictive of in vivo human response. Because ENMs vary widely in their physical and chemical characteristics, it is at present difficult to predict a priori their likely interaction with and toxicity toward biological systems. Moreover, it is likely that different cell types and target tissues will show unique sensitivities toward these materials. It is essential that comparative in vitro studies with a variety of different tissue types be conducted on a variety of physically and chemically different, but well characterized, nanomaterials of potential widespread use. In ongoing studies we are assessing the toxicity of quantum dot nanomaterials in air liquid interface cultures of respiratory epithelial cells, and human and mouse hepatocyte cultures. We have recently expanded these studies to include additional nanomaterials nominated for study by the NCNHIR consortium, as well as additional cell types that may represent additional important target organs for nanomaterials.

Selected Publications

(*Indicates students, post-doctoral fellows or research technologists under the direction of Dr. Eaton).

A. RESEARCH PAPERS IN REFEREED JOURNALS

  1. Eaton DL, Klaassen CD. Effects of acute administration of taurocholic and taurochenedeoxycholic acid on biliary lipid excretion. Proc Soc Exp Biol Med 151:198-202, 1975.
  2. Eaton DL, Poisner AM. Plasma pseudorenin in rats after alteration in the renin-angiotensin system. Proc Soc Exp Biol Med 154:6-8, 1977.
  3. Eaton DL, Klaassen CD. Carrier-mediated transport of ouabain in isolated hepatocytes. J Pharmacol Exp Ther 285:480-488, 1978.
  4. Iwamoto I, Eaton DL, Klaassen CD. Uptake of morphine and nalorphine by isolated rat hepatocytes. J Pharmacol Exp Ther 206:181-190, 1978.
  5. Eaton DL, Klaassen CD. Carrier-mediated transport of the organic cation procaineamide ethobromide by isolated rat liver parenchymal cells. J Pharmacol Exp Ther 206:595-606, 1978.
  6. Iga T, Eaton DL, Klaassen CD. Uptake of unconjugated bilirubin by isolated rat hepatocytes. Am J Physiol 236:C9-C14, 1979.
  7. Eaton DL, Klaassen CD. Effects of microsomal enzyme inducers on carrier-mediated transport systems in isolated rat hepatocytes. J Pharmacol Exp Ther 208:381-385, 1979.
  8. Eaton DL, Klaassen CD. Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin, kepone and polybrominated biphenyls on transport systems in isolated rat hepatocytes. Toxicol Appl Pharmacol 51:137-144, 1979.
  9. Eaton DL, Stacey NH, Wong KL, Klaassen CD. Dose-response effects of various metal ions on rat liver metallothionein, glutathione, heme oxygenase and cytochrome P-450. Toxicol Appl Pharmacol 55:393-402, 1980.
  10. Eaton DL. Biliary excretion of 2,4,5-trichlorophenoxyacetic acid in the rat. Toxicol Lett 14:175-181, 1982.
  11. Eaton DL, *Toal BF. Evaluation of the Cd/hemoglobin affinity assay for the rapid determination of metallothionein in biological tissues. Toxicol Appl Pharmacol 66:134-142, 1982.
  12. Eaton DL, *Toal BF. A simplified method of quantitating metallothionein in biological tissues. J Sci Total Environ 28:375-384, 1983.
  13. *Carpenter LA, Eaton DL. The disposition of 2,4-dichlorophenoxyacetic acid (2,4-D) in the rainbow trout, Salmo gairdneri. Arch Environ Contam Tox 12:162-173, 1983.
  14. *Stinson MD, Eaton DL. Concentrations of lead, cadmium, mercury and copper in the crayfish (Pacificasticus lenisculus) obtained from a lake receiving urban runoff. Arch Environ Contam Toxicol 12:693-700, 1983.
  15. Woods JS, Fowler BA, Eaton DL. Studies on the mechanisms of thallium-mediated inhibition of hepatic mixed function oxidase activity: Correlation with inhibition of NADPH Cytochrome c (P-450) reductase. Biochem Pharmacol 33:571-576, 1984.
  16. Woods JS, Eaton DL, *Lukens C. Studies on porphyrin metabolism in the kidney: Effects of trace metals and glutathione on renal uroporphyrinogen decarboxylase. Mol Pharmacol 26:336-341, 1984.
  17. Kalman D, Eaton DL, Schumacher RS, Covert D. Biological availability of lead in a paint aerosol. I. Physical and chemical characterization of a lead paint aerosol. Toxicol Lett 22:301-306, 1984.
  18. Eaton DL, Kalman DA, *Garvey D, Morgan M, Omenn GS. Biological availability of lead in a paint aerosol. 2. Absorption, distribution and excretion of intratracheally instilled lead paint particles in the rat. Toxicol Lett 22:307-313, 1984.
  19. Eaton DL. Short Communications: Effects of various trace metals on the binding of cadmium to rat hepatic metallothionein determined by the Cd/hemoglobin affinity assay. Toxicol Appl Pharmacol 78:158-162, 1985.
  20. *Monroe DH, *Holeski CJ, Eaton DL. Effects of single dose and repeated dose pretreatment with 2(3)-tert-butyl-4-hydroxyanisole (BHA) on the hepatobiliary disposition and covalent binding to DNA of Aflatoxin B1in the rat. Food Chem Toxicol 24:1273-1281, 1986.
  21. Eaton DL, *Richards JA. Kinetic evaluation of carrier-mediated transport of ouabain and taurocholic acid in isolated rat hepatocytes. Evidence for independent transport systems. Biochem Pharmacol 35:2721-2725, 1986.
  22. *Holeski CJ, Eaton DL, *Monroe DH, *Bellamy GM. Effects of phenobarbital on the biliary excretion of Aflatoxin P1-glucuronide and AflatoxinB1-S-glutathione in the rat. Xenobiotica 17:139-153, 1987.
  23. *Monroe DH, Eaton DL. Comparative effects of butylated hydroxyanisole (BHA) on the in vivo and in vitro biotransformation of Aflatoxin B1(AFB) in rat and mouse. Toxicol Appl Pharmacol 90:401-409, 1987.
  24. Geraci JP, Dunston SG, Jackson KL, Mariano MS, *Holeski C, Eaton DL. Bile loss in the acute intestinal radiation syndrome in rats. Rad Res 109:47-57, 1987.
  25. Eaton DL, *Monroe DH, *Bellamy G and Kalman DA. Identification of a novel dihydroxy-metabolite of aflatoxin B1formed both in vivo and in vitro in rats and mice. Chem Res Toxicol 1:108-114, 1988.
  26. Franzblau A, Rosenstock L and Eaton DL. Use of inductively coupled plasma-atomic absorption spectroscopy (ICP-AES) in screening for trace metal exposures in an industrial population. Environ Res 46:15-24, 1988.
  27. *Monroe DH and Eaton DL. Effects of modulation of hepatic glutathione on biotransformation and covalent binding of aflatoxin B1to DNA in the mouse. Toxicol Appl Pharmacol 94:118-127, 1988.
  28. *Ramsdell HS and Eaton DL. Modification of aflatoxin B1biotransformation in vitro and DNA binding in vivo by dietary broccoli in rats. J Toxicol Environ Health 25:269-275, 1988.
  29. Eaton DL, *Stapleton PL. Simultaneous determination of cytosolic glutathione S-transferase and microsomal epoxide hydrolase activity toward benzo[a]pyrene-4,5-oxide by high performance liquid chromatography. Analytl Biochem 178:153-158, 1989.
  30. *Ramsdell HS, Eaton DL. Species susceptibility to aflatoxin B1carcinogenesis: Comparative kinetics of biotransformation. Cancer Res 50:615-620, 1990.
  31. *Ramsdell HS, Eaton DL. Mouse liver glutathione S-transferase isoenzyme activity toward aflatoxin B1-8,9-epoxide and benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide. Toxicol Appl Pharmacol 105:216-225, 1990.
  32. Kavanagh TJ, Grossmann A, Jaecks EP, Jinneman JC, Eaton DL, Martin GM, Rabinovitch PS. Proliferative capacity of human peripheral blood lymphocytes sorted on the basis of glutathione content J Cell Physiol, 145:472-480, 1990.
  33. *Ramsdell HS, Parkinson A, Eddy C, Eaton DL. Bioactivation of aflatoxin B1by human liver microsomes. Role of cytochrome P450IIIA isoenzymes. Toxicol Appl Pharmacol 108:436-447, 1991.
  34. *Trenga CA, Kunkel DD, Eaton DL, Costa LG. Effect of styrene oxide on rat brain glutathione. Neurotoxicology 12:165-178, 1991.
  35. *Chen ZY, Eaton DL. Differential regulation of cytochrome P450 IIB1/2 by phenobarbital in hepatocellular hyperplastic nodules induced by aflatoxin B1or diethynitrosamine plus 2-acetylaminofluorene in male F344 rats. Toxicol Appl Pharmacol 111:132-144, 1991.
  36. *Borroz KI, *Ramsdell HS, Eaton DL. Mouse strain differences in glutathione S-transferase activity and aflatoxin B1biotransformation. Tox Lett 58:97-105, 1991.
  37. Rosenstock L, Keefer M, Daniell WE, McConnell R, Claypoole K, Barnhart S, Costa LG, Demers P, Eaton DL, Morris S, Murphy SD. Chronic central nervous system effects of acute organophosphate pesticide intoxication. The Lancet 338:223-227, 1991.
  38. *Anderson PN, Eaton DL, Murphy SD. Comparative metabolism of methyl parathion in intact and subcellular fractions of isolated rat hepatocytes. Fund Appl Toxicol 18:221-226, 1992.
  39. Heckbert SH, Weiss NS, Hornung SK, Eaton DL, Motulsky AG. Glutathione S-transferase and epoxide hydrolase activity in human leukocytes in relation to the risk of lung and other smoking-related cancers. J Nat’l Cancer Inst 84:414-422, 1992.
  40. *Buetler TM, Eaton DL. cDNA cloning, mRNA expression and induction of a-class glutathione S-transferases in mouse tissues. Cancer Res 52:314-318, 1992.
  41. *Hamel DM, *White C, Eaton DL. Determination of g-glutamylcysteine synthetase and glutathione synthetase activity by HPLC. Toxicology Methods 1:273-288, 1992.
  42. Kensler TW, Groopman JD, Eaton DL, Curphey TJ, Roebuck BD. Potent inhibition of aflatoxin-induced hepatic tumorigenesis by the monofunctional enzyme inducer 1,2-dithiole-3-thione. Carcinogenesis 13:95-100, 1992.
  43. *Chen ZY, Farin F, Omiecinski CO, Eaton DL Association between growth stimulation by phenobarbital and expression of cytochromes P450 1A1, 1A2, 2B1/2 and 3A1 in hepatic hyperplastic nodules in male F344 rats. Carcinogenesis 13:675-682, 1992.
  44. *Buetler TM, *Slone, D and Eaton, DL. Comparison of the aflatoxin B1-8,9-epoxide conjugating activities of two bacterially expressed alpha class glutathione S-transferase isozymes from mouse and rat, Biochem Biophys Res Commun 188:597-603, 1992.
  45. Kavanagh TJ, Grossman A, Jinneman JC, Kanner SB, *White CC, Eaton DL, Ledbetter JA, Rabinovitch PS. Glutathione depletion by 1-chloro-2,4-dinitrobenzene inhibits T-cell receptor-stimulated transmembrane signal transduction in purified subsets of human peripheral blood lymphocytes. Toxicol Appl Pharmacol. 119:91-99, 1993.
  46. *Buetler TM, Eaton, DL. Glutathione S-transferases: amino acid sequence comparison, classification and phylogenetic relationship. Environ Carcin Ecotox Revs, C10:181-203, 1992.
  47. Daniell W, Barnhart S, Demers P, Costa LG, Eaton DL, Miller M, Rosenstock L. Neuropsychological performance among agricultural pesticide applicators. Env Res 59:217-228, 1992.
  48. Karr C, Demers P, Costa LG, Daniell WE, Barnhart S, Miller M, Gallagher G, Horstman SW, Eaton D, Rosenstock L. Organophosphate pesticide exposure in a group of Washington state orchard applicators. Env Res 59:229-237, 1992.
  49. Hulla JE, *Chen ZY, Eaton, DL. Aflatoxin B1-induced rat hepatic hyperplastic nodule do not exhibit a site-specific mutation within the p53 gene. Cancer Res 53:9-11, 1993.
  50. Nazar-Stewart V, Motulsky AG, Eaton DL, White, EJ, Hornung, SK, Leng, Z-T, Stapleton, P and Weiss, NS. The Glutathione S-Transferase mu polymorphism as a marker for susceptibility to lung carcinoma. Cancer Res.53: 2313-2318, 1993.
  51. *Chen Z-Y, Eaton DL. Association between responsiveness to phenobarbital induction of CYP2B1/2 and 3A1 in rat hepatic hyperplastic nodules and their zonal origin. Environ Health Perspect., 101 (Suppl. 5): 185-190, 1993.
  52. *Chen, ZY, *White, CC and Eaton DL. Decreased expression of cytochrome P450 mRNA and related steroid hydroxylation activities in hepatic hyperplastic nodules in male F344 rats. Toxicol Appl Pharmacol. 123: 151-159, 1993.
  53. Davis, MA, Wallig, MA, Eaton DL, *Borroz, KI and Jeffrey, EH. Differential effect of cyanohydroxybutene on glutathione synthesis in liver and pancreas of male rats. Toxicol. Appl. Pharmacol.,123: 257-264, 1993.
  54. Eaton DL, *Hamel DM. Increase in g-glutamylcysteine activity as a mechanism for butylated hydroxyanisole-mediated elevation of hepatic glutathione. Toxicol Appl Pharmacol, 126: 145-149, 1994.
  55. *Borroz KI, *Buetler TM, Eaton DL. Modulation of g-glutamylcysteine synthetase mRNA expression by butylated hydroxyanisole. Toxicol Appl Pharmacol 126: 150-155, 1994.
  56. *Gallagher EP, Wienkers LC, *Stapleton PL, Kunze KL, Eaton DL. Role of CYP4501A2 and CYP4503A4 in the bioactivation of aflatoxin B1(AFB1) by human liver microsomes. Cancer Research 54: 1-8, 1994.
  57. *Chen Z-Y, Liu Y-F, *He C-Y, *White CC, Eaton DL. Inhibition of cell proliferation by ciprofibrate in GST - Positive rat hepatic hyperplastic nodules, Cancer Research 54: 2622-2629, 1994.
  58. Kavanagh TJ, Raghu G, *White CC, Martin GM, Rabinovitch PS, Eaton DL. Enhancement of glutathione content in glutatione synthetase-deficient fibroblasts from a patient with 5-oxoprolinuria via metabolic cooperation with normal fibroblasts. Exp. Cell Res. 212: 69-76, 1994.
  59. *Van Ness KP, *Buetler TM, Eaton DL. Enzymatic characteristic of chimeric mYc/rYC1 glutathione S-transferases Cancer Res. 54 (17): 4573-4575, 1994.
  60. *Gallagher EP, Eaton DL. In vitro biotransformation of aflatoxin B1(AFB1) in channel catfish liver. Toxicology and Applied Pharmacology, 132:82-90, 1995.
  61. *Slone DH,*Gallagher EP, *Ramsdell HS, Rettie AE, Stapleton PL, Berlad LG, Eaton DL. Human variability in hepatic glutathione S-transferase mediated conjugation of aflatoxin B1-epoxide and other substrates. Pharmacogenetics, 5:224-233, 1995.
  62. Eaton DL, *Gallagher EP, *Bammler T, Kunze KL. Role of Cytochrome P450 1A2 in chemical carcinogenesis: Implications for human variability in expression and enzyme activity. Pharmacogenetics, 5:259-274, 1995.
  63. *Heinonen JT, Fisher R, Brendel K, Eaton DL. Determination of aflatoxin B1metabolism and binding to hepatic macromolecules in human precision liver slices. Toxicology and Applied Pharmacology, 136: 1-7, 1996.
  64. *Gallagher EP, *Buetler TM, *Wang CH, *Stapleton PL, *Stahl DL, Eaton DL. The effects of diquat and ciprofibrate on mRNA expression and catalytic activities of hepatic drug metabolizing and antioxidant enzymes in rat liver. Toxicol. Applied Pharmacol., 134:81-91, 1995.
  65. *Buetler TM, *Gallagher EP, *Wang C-H, *Stahl DL, Hayes JD, Eaton, DL. Induction of phase I and phase II drug metabolizing enzyme mRNA, protein and activity by BHA, ethoxyquin and oltipraz. Toxicology and Applied Pharmacology, 135:45-57, 1995.
  66. *Gallagher EP, *Stapleton PL, *Slone DH, Schlenk D, Eaton DL. Channel catfish Glutathione S--transferase isoenzyme activity toward (+)-anti-benzo[a]pyrene-trans-7,8,-dihydrodiol-9,10-epoxide. Aquatic Toxicology, 34: 135-150, 1996.
  67. *Chen ZY, *White CC, *He C-Y, Liu Y-F, Eaton DL. Zonal differences in DNA synthesis activity and CYP gene expression in livers of male F344 rats treated with five nongenotoxic carcinogens. J. Environ. Pathol. Toxicol. Oncol. 14: 83-99, 1995.
  68. *Buetler TM, *Bammler, T., Hayes JD, and Eaton DL. Oltipraz-mediated changes in aflatoxin B1- biotransformation in rat liver: Implications for human chemointervention. Cancer Research, 56: 2306-2313, 1996.
  69. *Heinonen JT, Sidhu JS, Reilly MT, Farin FM, Omiecinski CJ, Eaton DL, Kavanagh TJ. Assessment of regional cytochrome P-450 activities in precision-cut rat liver slices using resorufin substrates and fluorescence confocal laser cytometry. Environmental Health Perspectives, 104: 536-543, 1996.
  70. *Gallagher, EP, Kunze KL, *Stapleton PL, Eaton, DL. The kinetics of aflatoxin B1oxidation by human cDNA-expressed and human liver microsomal cytochromes P450 1A2 and 3A4. Toxicol. Applied Pharmacol., 141(2):595-606, 1996.
  71. *McHugh TE, Atkins WM, Racha JK, Kunze KL, Eaton DL. Binding of the Aflatoxin-glutathione Conjugate to Mouse Glutathione S-Transferase A3-3 is Saturated at Only On Ligand per Dimer. J.Biol. Chem. 271: 27470-74, 1996.
  72. Kelly, JD, Eaton, DL, Guengerich, FP and Coulombe, RA, Jr. Aflatoxin B1activation in human lung. Toxicol. Appl. Pharmacol. 144: 88-95, 1997.
  73. Potter AJ, Grossmann A, Rabiovitch PS, Eaton DL and Kavanagh TJ. The effect of in vitro phorone exposure on glutathione content and T cell antigen receptor (CD3)-stimulated calcium mobilization in murine splenic T lymphocytes. Toxicol. In Vitro , 11:355-363, 1997.
  74. Neal, GE, Eaton, DL, Judah, DJ and Verma, A. Metabolism and toxicity of aflatoxins M1and B1in human-derived in vitro systems. Toxicol. Appl. Pharmacol., 151:152-158, 1998.
  75. *Gallagher EP, Sheehy KM, *Janssen PL, Eaton DL and Collier TC. Isolation and cloning of homologous glutathione S-transferases cDNAs from English sole and starry flounder. Aquat. Toxicol. 44: 171-182, 1998.
  76. *Van Ness KP, *McHugh TM, *Bammler TK and Eaton DL Identification of amino acid residues essential for high aflatoxin B1-8,9-epoxide conjugation activity in Alpha class glutathione S-transferases through site-directed mutagenesis. Toxicol. Appl. Pharmacol., 152: 166-174, 1998.
  77. Tonge RP, *Kelly EJ, Bruschi SA, Kalhorn T, Eaton DL, Nebert DW and Nelson SD. Role of CYP1A2 in the hepatotoxicity of acetaminophen: Investigations using Cyp1a2 null mice. Toxicol. Appl. Pharmacol. 153: 102-108, 1998.
  78. Thompson SA, White CC, Krejsa CM, Diaz D, Woods JS, Eaton DL, Kavanagh TJ. Induction of glutamate-cysteine ligase (gamma-glutamylcysteine synthetase) in the brains of adult female mice subchronically exposed to methylmercury. Toxicol Lett. 110:1-9, 1999.
  79. *Gallagher EP, Sheehy KM, Janssen PJ, Eaton DL and Collier TK. Isolation and cloning of homologous glutathione S-transferase cDNAs from English sole and starry flounder liver. Aquatic Toxicol. 44: 171-182, 1999.
  80. Eaton DL. Biotransformation enzyme polymorphisms and pesticide susceptibility. NeuroTox, 21: 101-11, 2000.
  81. *Bammler, TK, *Slone, DH and Eaton, DL. Effects of dietary oltipraz and ethoxyquin on aflatoxin B1 biotransformation in non-human primates, Toxicol. Sci., 54: 30-41, 2000.
  82. Vasiliou, V, *Buetler, T, Eaton, DL and Nebert, DW. Comparison of oxidative stress response parameters in newborn mouse liver versus SV40-transformed hepatocyte cell lines, Biochemical Pharmacol. 59: 703-12, 2000.
  83. Sweeney C, Farrow DC, Schwartz SM, Eaton DL, Checkoway H, Vaughan TL. Glutathione S-transferase M1, T1, and P1 Polymorphisms as Risk Factors for Renal Cell Carcinoma: a Case-Control Study. Cancer Epi. Biomark. Prev., 9: 449-54, 2000.
  84. *Wang CH, *Bammler, TK, *Ou, YY, *Kelly EJ and Eaton DL. Mu-class GSTs Are Responsible for Aflatoxin B1-8, 9-epoxide Conjugating Activity in the Non-human Primate Macaca fascicularis Liver, Toxicol. Sci. 56: 26-36, 2000.
  85. Thompson SA, White CC, Krejsa CM, Eaton DL, Kavanagh TJ.Modulation of glutathione and glutamate-L-cysteine ligase by methylmercury during mouse development. Toxicol Sci. 57:141-6, 2000.
  86. Johnson DB, Eaton DL, Wahl PW, Gleason C, Public health nutrition practice in the United States, J Am Diet Assoc 101: 5: 529-34, 2001.
  87. Cheesman MJ, Kneller MB, *Kelly EJ, Thompson SJ, Yeung CK, Eaton DL, Rettie AE.Purification and characterization of hexahistidine-tagged cyclohexanone monooxygenase expressed in Saccharomyces cerevisiae and Escherichia coli. Protein Expr Purif. 21:81-6, 2001.
  88. *Kelly EJ, Erickson KE, Sengstag C and Eaton, DL. Expression of human microsomal epoxide hydrolase in Saccharomyces cerevisiae reveals a functional role in aflatoxin B1 detoxification.
    Toxicol Sci 65:35-42, 2002.
  89. *Bammler, TK *Abel EL and Eaton DL. Metabolism of methyl parathion by glutathione S-transferases in vitro. Chemico-Biol. Interact. 133: 231-33, 2001.
  90. *Wang C, *Bammler, TK and Eaton DL. Complementary DNA cloining, protein expression and characterization of alpha-class GSTs from Macaca Fasicularis Liver, Toxicol. Sci., 70: 20-26, 2002.
  91. *Kelada SN, *Stapleton PL, *Farin FM, *Bammler TK, Eaton DL, Smith-Weller T, Franklin GM, Swanson PD, Longstreth WT Jr and Checkoway H. Glutathione S-transferase M1, T1 and P1 polymorphisms and Parkinson’s Disease. Neurosci. Lett. 337: 5-8, 2003.
  92. Sweeney C, Nazar-Stewart V, *Stapleton PL, Eaton DL, Vaughan TL. Glutathione S-transferase M1, T1, and P1 polymorphisms and survival among lung cancer patients. Cancer Epi. Biomark. Prev. 12: 527-33, 2003.
  93. Nazar-Stewart V, Vaughan TL, *Stapleton P, Van Loo J, Nicol-Blades B, Eaton DL. A population-based study of glutathione S-transferase M1, T1 and P1 genotypes and risk for lung cancer. Lung Cancer 40:247-58, 2003.
  94. *Abel EL, *Bammler TK and Eaton DL. Biotransformation of methyl parathion by glutathione S-transferases. Toxicol. Sci. 79(2):224-32, 2004.
  95. *Abel EL, Opp SM, Verlinde CLMJ;* Bammler TK and Eaton DL. Characterization of atrazine biotransformation by human and murine Glutathione S-transferases, Toxicol. Sci, 80:230-8, 2004.
  96. *Gross-Steinmeyer K, *Stapleton P L, Liu F, *Tracy J H, *Bammler T K, Quigley S D, *Farin F M, Buhler DR, Safe S H , Strom S C, and Eaton D L. Phytochemical-induced changes in gene expression of carcinogen metabolising enzymes in cultured human primary hepatocytes. Xenobiotica, 34: 619-632, 2004.
  97. *Abel EL, Lyon RP, *Bammler TK, Lau SS, Monks TJ., and Eaton DL Estradiol metabolites as isoform-specific inhibitors of human Glutathione S-transferases. Chem-Biol. Interact 151(1):21-32, 2004.
  98. *Gross-Steinmeyer K, Stapleton PL, Tracy JH,. *Bammler TK, Lehman T, Strom SC and Eaton DL. Influence of Matrigel overlay on constitutive and inducible expression of nine genes encoding drug metabolizing enzymes in primary human hepatocytes. Xenobiotica 35(5):419-38, 2005.
  99. *Guo YY, Breeden LL, Zarbl H, Preston BD and Eaton DL. Expression of a human cytochrome P450 in yeast permits analysis of pathways for response to and repair of aflatoxin-induced DNA damage. Mol. Cellular Biol. 25(4): 5823-33, 2005.
  100. *Guo YY, Breeden LL, Fan W, Zhao LP, Eaton DL and Zarbl H. Characterization of DNA repair pathways following aflatoxin B1 treatment in Saccharmocyes cerivisiae expressing human cytochrome P4501A2. Mutat. Res., 593(1-2):121-42, 2006.
  101. Sieh W, Edwards KL, Fitzpatrick AL, Srinouanprachanh SL, *Farin F, Monks SA, Kronmal RA and Eaton DL. Genetic susceptibility to prostate cancer: Prostate-specific antigen and its interaction with the androgen receptor. Cancer Causes and Controls, 17(2):187-97, 2006.
  102. *Peterson S, Lampe JW, *Bammler TK, *Gross-Steinmeyer K and Eaton DL. Apiaceous vegetable constituents inhibit human cytochrome P-450 1A2 (CYP1A2) activity and hCYP1A2-mediated mutagenicity of aflatoxin B1. Food Chem Toxicol. 44(9):1474-84, 2006.
  103. *Kelada SN, Checkoway H, Kardia SL, Carlson CS, Costa-Mallen P, Eaton DL, Firestone J, Powers KM, Swanson PD, Franklin GM, Longstreth WT Jr, Weller TS, Afsharinejad Z, Costa LG. 5' and 3' region variability in the dopamine transporter gene (SLC6A3), pesticide exposure and Parkinson's disease risk: a hypothesis-generating study. Hum Mol Genet. 15(20):3055-62, 2006.
  104. *Zhou C, *Poulton EJ, Grun F, *Bammler TK, Blumberg B, Thummel KE, Eaton DL. The dietary isothiocyanate sulforaphane is an antagonist of the human steroid and xenobiotic nuclear receptor. Mol Pharmacol. 71(1):220-9, 2007.
  105. Biggs ML, Davis MD, Eaton DL, Weiss NS, Barr DB, Doody DR, Fish S, Needham LL, Chen C, Schwartz SM. Serum organochlorine pesticide residues and risk of testicular germ cell carcinoma: a population-based case-control study. Cancer Epidemiol Biomarkers Prev. 17(8):2012-8, 2008.
  106. Shi M, Bradner J, *Bammler TK, Eaton DL, Zhang J, Ye Z, Wilson AM, Montine TJ, Pan C, Zhang J. Identification of glutathione S-transferase pi as a protein involved in Parkinson disease progression. Am J Pathol.;175(1):54-65, 2009.
  107. Peterson S, Schwarz Y, . Li, SS, Lin Li, L King IB, Chen C, Eaton DL, Potter JD and Johanna W. Lampe JW. CYP1A2, GSTM1, and GSTT1 polymorphisms and diet effects on CYP1A2 activity in a crossover feeding trial, Cancer Epidemiol Biomarkers Prev., 18(11):3118–25, 2009.
  108. *Gross-Steinmeyer, *Stapleton PL, *Tracy JH, Bammler TK, Strom SC, Buhler, DR and Eaton DL. Modulation of aflatoxin B1-mediated genotoxicity in primary cultures of human hepatocytes by diindolylmethane, curcumin and xanthohumols. Toxicol. Sci 112(2), 303–310, 2009.
  109. *Gross-Steinmeyer K, *Stapleton PL, *Tracy JH, *Bammler TK, Strom SC, Eaton DL. Sulforaphane- and Phenethyl isothiocyanate-Induced Inhibition of Aflatoxin B1-Mediated Genotoxicity in Human Hepatocytes: Role of GSTM1 genotype and CYP3A4 Gene Expression. Toxicol Sci. 116(2):422-32, 2010.
  110. McConnachie LA, White CC, Botta D, Zadworny ME, Cox DP, Beyer RP, Hu X, Eaton DL, Gao X, Kavanagh TJ. Heme oxygenase expression as a biomarker of exposure to amphiphilic polymer-coated CdSe/ZnS quantum dots. Nanotoxicology. In press. 2012.
  111. *Poulton EJ, Levy L, Lampe JW, Danny D. Shen DD, Tracy J, Shuhart MC, Thummel KT and Eaton DL.  Sulforaphane is not an effective antagonist of the human pregnane X-receptor in vivo.  Toxicol Appl Pharmacol. 2013 Jan 1;266(1):122-31. doi: 10.1016/j.taap.2012.10.029. Epub 2012 Nov 12; PMID: 23153560
  112. *Smith WE, Brownell J, White CC, Afsharinejad Z, Tsai J, Hu X, Polyak SJ, Gao X, Kavanagh TJ and Eaton DL. In vitro toxicity assessment of amphiphillic polymer-coated CdSe/ZnS quantum dots in two human liver cell models. ACS Nano. 2012 Nov 27;6(11):9475-84. doi: 10.1021/nn302288r. Epub 2012 Oct 18.  PMID: 23039050
  113. McConnachie, L A, White CC, Botta D, Zadworny ME, Cox DP, Beyer RP, Hu X, Eaton DL, Gao X and Kavanagh TJ.   Heme oxygenase expression as a biomarker of exposure to amphiphilic polymer-coated CdSe/ZnS quantum dots Nanotoxicology, in press, 2012. 
  114. Wang Z, Lin YS, Dickmann LJ, Poulton EJ, Eaton DL, Lampe JW, Shen DD, Davis CL, Shuhart MC, Thummel KE. Enhancement of hepatic 4-hydroxylation of 25-hydroxyvitamin D(3) through CYP3A4 induction in vitro and in vivo: Implications for drug-induced osteomalacia. J Bone Miner Res. 2012 Dec 4. doi: 10.1002/jbmr.1839. [Epub ahead of print] PMID: 23212742

*Student, Post-doc or Research Scientist reporting to Dr. Eaton.

B. BOOKS

Eaton DL, Groopman JD, eds. The Toxicology of Aflatoxins: Human Health, Veterinary and Agricultural Significance. Academic Press, New York, pp.544, 1994.

Costa, LG and Eaton, DL, eds, Gene-Environment Interactions: The Fundamentals of Ecogenetics, Wiley Press, New York, 2006.

C. BOOK CHAPTERS, PROCEEDINGS & PUBLISHED REVIEW PAPERS:

  1. Klaassen CD, Eaton DL, Cagen SZ. Hepatobiliary disposition of xenobiotics. In: Drug Metabolism Reviews. JW Bridges and LF Chasseaud, eds. London: John Wiley and Sons, 1981, p. 1-75.
  2. Eaton DL, *Ramsdell HR, *Monroe DH. Biotransformation as a determinant of species susceptibility to aflatoxin B1: in vitro studies in rat, mouse, monkey and human liver. In: Cellular and Molecular Mode of Action of Selected Microbial Toxins in Foods and Feeds, A Pohland, JL Richard, eds. Pergamon Press, New York, pp. 275-288, 1990.
  3. Klaassen CD, Eaton DL. Principles of Toxicology. In: Casarett and Doull's Toxicology: The Basic Science of Poisons, Fourth edition, MO Amdur, J Doull and CD Klaassen, eds., Pergammon Press, New York, pp. 12-49, 1991.
  4. Omenn GS, Omiecinski CJ, Eaton DL. Ecogenetics of chemical carcinogens. In: Biotechnology and Human Genetic Predisposition to Disease, Cantor C, Caskey T, Hood L, Kamely D, Omenn GS, Editors, Wiley-Liss Inc, New York, pp. 81-93, 1990.
  5. Eaton DL and Cherian MG. Determination of metallothionein in tissues by the Cadmium/Hemoglobin affinity assay. In: Methods in Enzymology, Metallobiochemistry, Riordan J, Valle B, Editors, Academic Press, New York, Chp. 13, pp. 83-88, 1991.
  6. Eaton DL, *Ramsdell HS. Species and diet related differences in aflatoxin biotransformation, In: Handbook of Applied Mycology, vol. 5, D. Bhatnagar, editor, Marcel Dekker, Inc., New York, Chap. 6, pp. 157-182, 1992.
  7. Eaton DL, Robertson WO. Toxicology. In: Textbook of Occupational and Environmental Medicine, Rosenstock L, Cullen M, eds. WB Saunders Company, New York, chapter 8, pp. 116-149, 1994.
  8. Eaton DL, *Ramsdell HS, Neal G, Biotrasformations of Aflatoxins, in Eaton DL, Groopman JD, eds. The Toxicology of Aflatoxins: Human Health, Veterinary and Agricultural Significance. Chp. 5, Academic Press, Orlando, pp.45-72, 1994.
  9. *Gallagher E, Kavanagh TJ, Eaton DL. Glutathione, Oxidized Glutathione and Mixed Disulfides in Biological Samples, IN: Methods in Toxicology, Vol. II: In Vitro Toxicity Indicators, CA Tyson, JM Frazier, eds., Academic Press, San Diego, pp. 349 - 366, 1994.
  10. Eaton DL, *Van Ness K, *Buetler, TM. GST-Mediated Protection Against Carcinogens: Aflatoxin B1detoxification as an example. In Structure and Function of Glutathione S-Transferases, K. Tew, C.B. Pickett, T.J. Mantle, B. Mannervik and J.D. Hayes, ed., CRC Press, London, pp. 187-198, 1993.
  11. Eaton DL, Grossmann A, Kavanagh TJ. Glutathione Biosynthesis as a Biomarker of Toxic Exposures, Pacific Basin Conference on Hazardous Wastes, 1993.
  12. Eaton DL, and *Gallagher EP. Mechanism of Aflatoxin Carcinogenesis. In Ann Rev Pharmacol Toxicol 34: 135-172, 1994.
  13. Eaton, DL and *Hill, J. Human Effects of Hazardous Materials, In: Ostler, N (ed.) Handbook of Hazardous Materials, Vo. 1, Prentice Hall, Columbus, Ohio,, pp. 81-118, 1996.
  14. Eaton DL, and Klaassen, CD. Principles of Toxicology, IN: Casarett & Doull’s Toxicology: The Basic Science of Poisons, 5th edition, ed. C.D. Klaassen, chp. 2, pp. 13-33, McGraw-Hill, New York, 1996.
  15. Eaton, DL and *Gallagher, EP. Introduction to the Principles of Toxicology, In: Comprehensive Toxicology, IG Sipes, CA McQueen and AJ Gandolfi, Editors, Elsevier Sciences, Vol. 1, General Principles, James Bond, Vol. Ed.; Chap. 1, pp. 1-38, 1997.
  16. Eaton, DL and Heinonen, J. Aflatoxins, In: Comprehensive Toxicology, IG Sipes, CA McQueen and AJ Gandolfi, Editors; Vol. 09, Liver and Gastrointestinal Systems, RS McCusky and DL Earnest, Vol. Editors, Elsevier Sciences, Chap. 26, pp. 407-422, 1997.
  17. Miller MS, McCarver DG, Bell DA, Eaton DL and Goldstein JA. Symposium Overview: Genetic Polymorphisms in Human Drug Metabolic Enzymes. Fund. Appl. Toxicol. 40: 1-14, 1997
  18. Eaton, DL, Farin, F, Omiecinski, CJ and Omenn, GS. Genetic Susceptibility, In: Environmental and Occupational Medicine, ed. WN Rom, 3rd Edition, Chp. 17, Little, Brown & Co., pp. 209-221, 1998.
  19. White, CC, Kresja, CM, Eaton, DL and Kavanagh, TJ. HPLC-Based assays for enzymes of glutathione biosynthesis. Current Protocols in Toxicology, Chp. 6.5, 1999.
  20. Eaton, DL and Bammler, TK. Concise Review of the Glutathione S-Transferases and Their Significance to Toxicology. Toxicol. Sci., 49: 156-164, 1999.
  21. Patterson RE, Eaton DL, Austin MA, Kristal AR and Potter JD. The Genetic Revolution: Change and Challenge for the Profession of Dietetics. J. Am. Dietic Assoc. 99: 1412-20, 1999.
  22. Eaton, DL and *Bammler, TK. Glutathione S-Transferases, IN: Metabolic Drug Interactions, R. H. Levy, K.E. Thummel, W.F. Trager, P.H. Hansten and M. Eichelbaum, Editors, Chapter 13, Lippincott-Raven, Philadelphia, 2000.
  23. Eaton, DL. Aflatoxin Biotransformation and Toxicology, IN: Molecular and Applied Aspects of Oxidative Metabolizing Enzymes, E. Arinc , JB Schenkman and E Hodgson, Editors, NATO ASI Series A: Life Sciences, Vol. 303, Chapter 14, pp. 195-209, Plenum Publishers, New York, 1999.
  24. Eaton DL, and Klaassen, CD. Principles of Toxicology, IN: Casarett & Doull’s Toxicology: The Basic Science of Poisons, 6th edition, ed. C.D. Klaassen, chp. 2, pp. 11-34, McGraw-Hill, New York, 2001.
  25. Eaton, DL, *Bammler, TK and *Kelly EJ. Interindividual differences in response to chemoprotection against aflatoxin-induced hepatocarcinogenesis: implications for human biotransformation enzyme polymorphisms. In: Biologically Reactive Intermediates VI., chemical and biological mechanisms in susceptibility to and prevention of environmental diesease; (Ed. PM Dansett, R Snyder, M. Delagorge et. al)., pp. 559-576, Kluwer Academic/Plenum Publishers; Adv Exp Med Biol. 500:559-76, 2001.
  26. Eaton DL, Robertson WO. Toxicology. In: Textbook of Occupational and Environmental Medicine, Rosenstock L, Cullen M, eds. WB Saunders Company, New York, chapter 8, pp. 2nd Edition, 2004.
  27. *Kelada, SN, Eaton DL, Wang SS Rothman NR and Khoury MJ. The role of genetic polymorphisms in environmental health. Environ. Health Perspect. 111: 1055-64, 2003.
  28. *Kelada, SN, Eaton DL, Wang SS Rothman NR and Khoury MJ. Applications of human genome epidemiology to environmental health, In: Khoury, M (Editor), Human Genome Epidemiology: A Scientific Foundation for Using Genetic Information to Improve Health and Prevent Disease, Chp. 9, Oxford University Press, London, 2003.
  29. Eaton DL and Klaassen, CD. Principles of Toxicology, In: ( CD Klaassen and JB Watkins III, eds) Casarett & Doull’s Essentials of Toxicology, Chp. 2, pp. 6-21, McGraw-Hill, 2003.
  30. *Peterson, S, Lampe, JW and Eaton DL. Genetic Susceptibility to dietary carcinogens. In: D. Watson, Editor) Pesticide, Veterinary and other Residues in Food, Woodhead Publishing, London, Chp. 2, 2004.
  31. Eaton, DL Glutathione S-Transferases as Putative Host Susceptibility Genes for Cancer Risk in Agricultural Workers. J. Biochem. Mol. Toxicol. 19(3): 1-2, 2005.
  32. *Smith H, *Bammler TK and Eaton DL. Polymorphisms in xenobiotic conjugation, in: Costa LG and Eaton DL (ed). Gene-Environment Interactions; The Fundamentals of Ecogenetics, Wiley Press, Chp. 8, 2006.
  33. Eaton DL, Gallagher E, Hooper M, Schlenk D, Schmeider P and Thompson C. Species Differences in Response to Toxic Substances: Shared Pathways of Toxicity — Value and Limitations of Omics Technologies to Elucidate Mechanism or Mode of Action. Chapter 3, IN: Emerging Molecular and Computational Approaches for Cross-Species Extrapolations, SETAC-Pellston Workshop, 2006.
  34. Eaton DL and Gilbert SG. Principles of Toxicology, IN: Casarett and Doull’s Toxicology: The Basic Science of Poisons, 7th Edition, Chp. 2, McGraw-Hill Publishers, New York, 2007.
  35. Eaton DL, Daroff RB, Autrup H, Bridges J, Buffler P, Costa LG, Coyle J, McKhann G, Mobley WC, Nadel L, Neubert D, Schulte-Hermann R, Spencer PS. Review of the toxicology of chlorpyrifos with an emphasis on human exposure and neurodevelopment. Crit Rev Toxicol. 2008;38 Suppl 2:1-125.
  36. Gilbert, SG and Eaton, DL. Ethical, Legal, Social and Professional Issues in Toxicology, IN: General and Applied Toxicology, 3rd Edition (B. Ballantyne, TC Marrs & T Swersen, Editors), Part 11, Chp. 116; Wiley, 2009.
  37. Eaton, DL and Gallagher, EP. Introduction to the Principles of Toxicology, In: Comprehensive Toxicology, IG Sipes, CA McQueen and AJ Gandolfi, Editors, Elsevier Sciences, Vol. 1, General Principles, James Bond, Vol. Ed.; 2nd Edition Chap. 1, 2010.
  38. Eaton, DL, *Biema, K, Bammler TK, Riley R and Voss K. Hepatotoxic Mycotoxins. In: Comprehensive Toxicology, IG Sipes, CA McQueen and AJ Gandolfi, Editors; Vol. 09, Liver and Gastrointestinal Systems, RS McCusky and DL Earnest, Vol. Editors, Elsevier Sciences, Chap. 26, 2010.
  39. *Peck EC and Eaton, DL. Genetic Polymorphism and Susceptibility to Pesticides, IN: Handbook of Pesticide Toxicology, Third Edition (Ed, Robert Krieger), Elsevier, 2010.
  40. Eaton DL and Gilbert SG. Principles of Toxicology, IN: Casarett and Doull’s Toxicology: The Basic Science of Poisons, 8th Edition, Chp. 2, McGraw-Hill Publishers, New York, 2012.
Review date: 
3/6/2013